Can You Use a Pregnancy Test on a Dog? The Surprising Truth About Canine Pregnancy Testing

September 25, 2025 |

No, human pregnancy tests are not suitable for dogs. Human pregnancy tests are designed to detect human chorionic gonadotropin (hCG), a hormone produced during human pregnancy. Dogs do not produce hCG, so using these tests on them would not provide accurate results.

If you suspect that your dog might be pregnant, its essential to do a home pregnancy test or consult with a veterinarian for proper diagnosis and guidance. Veterinarians have access to various methods and specialized tests to confirm pregnancy in dogs. One such option is the Bellylabs pregnancy test for dogs – also suitable for a home-use– which measures the relaxin hormone.

The relaxin hormone is produced by the placenta during pregnancy, making it a reliable indicator of dog pregnancy. Unlike human pregnancy tests, the Bellylabs test is specifically designed to detect relaxin in dogs, ensuring accurate results.

Attempting to use a human pregnancy test on a dog is not a reliable or appropriate method for determining pregnancy in dogs and could lead to inaccurate results and confusion about your dogs health. Always seek professional veterinary advice and consider using specialized tests like the Bellylabs pregnancy test for dogs when assessing your pets well-being during pregnancy. This will help ensure the best care for your pregnant dog and her potential puppies.

Have you ever wondered if those drugstore pregnancy tests could work on your potentially pregnant pooch? Maybe you’ve found yourself staring at that little pink stick, contemplating whether it might save you a trip to the vet. I totally get it – as a dog owner myself, I’ve been there!

Today, I’m tackling one of the most common questions pet owners have when they suspect their dog might be expecting: can you use a human pregnancy test on a dog?

The Short Answer: Nope, It Just Won’t Work

Let me save you some time and money right off the bat – human pregnancy tests cannot detect pregnancy in dogs This isn’t just my opinion; it’s a scientific fact backed by veterinarians and reproductive specialists worldwide.

But why exactly won’t it work? And what should you do instead? Let’s dig deeper.

Why Human Pregnancy Tests Don’t Work for Dogs

The reason is actually pretty fascinating and comes down to fundamental biological differences between humans and canines

One veterinarian I spoke with compared using a human pregnancy test on a dog to “trying to use a house key to start your car – they’re just made for completely different locks.” That analogy really stuck with me!

Dangers of Relying on Human Pregnancy Tests for Dogs

Using human pregnancy tests for your dog isn’t just ineffective – it can actually cause problems:

  1. False negatives: Since dogs don’t produce hCG, the test will always be negative – even if your dog is definitely pregnant.

  2. Delayed care: Believing a negative result might prevent you from getting proper veterinary care during critical early stages of pregnancy.

  3. Missed breeding opportunities: If you’re intentionally breeding, you might miss the chance to try again if you falsely believe your dog is pregnant.

  4. Nutritional concerns: Pregnant dogs need specific nutritional support, especially in the second half of gestation. Missing this window can impact puppy development.

I once heard about a Labrador breeder who relied on a negative human pregnancy test, only to discover her dog was actually carrying a single large puppy. This singleton pregnancy required emergency intervention because she missed the window for proper monitoring. Yikes!

Early Signs Your Dog Might Be Pregnant

While you can’t use a human pregnancy test, there are some physical and behavioral changes that might suggest your dog is expecting. Just remember – these signs aren’t definitive and can also appear with pseudopregnancy (false pregnancy).

Physical Signs (Usually After 3 Weeks)

  • Slight nipple enlargement and pinkening
  • Gradual weight gain, especially in the abdomen
  • Changes in appetite (possibly including brief “morning sickness” around days 21-25)
  • Decreased energy or tiring more easily

Behavioral Changes

  • Nesting behavior (arranging bedding, seeking quiet spaces)
  • Changes in affection level (either more clingy or more independent)
  • Less enthusiasm for play or walks
  • Changes in interactions with other dogs

Remember that most dogs show virtually no reliable signs before 3 weeks after breeding, and even then, they remain inconsistent and could indicate pseudopregnancy instead.

The Real Ways to Confirm Dog Pregnancy

So what should you do instead of grabbing that human pregnancy test? Here are the reliable, vet-approved methods to confirm pregnancy in dogs:

1. Relaxin Blood Test

  • When it works: Around day 28+ after breeding
  • How it works: Detects relaxin, the pregnancy-specific hormone in dogs
  • Pros: Specifically detects true pregnancy, distinguishes from pseudopregnancy
  • Cons: Cannot assess fetal viability or count puppies

2. Ultrasound

  • When it works best: Day 25-30 post-breeding
  • How it works: Uses sound waves to visualize the uterus and developing puppies
  • What you’ll see: Gestational sacs, embryos, and even fetal heartbeats
  • Pros: Early confirmation, viability assessment, initial litter size estimate
  • Cons: Less accurate for precise puppy counting than X-rays

3. X-Ray (Radiography)

  • When it works: After day 42, but most accurate around days 55-60
  • What it shows: Fetal skeletons (skulls and spines)
  • Main benefit: Accurate puppy count before whelping
  • Why it matters: Knowing exactly how many puppies to expect is crucial for safe delivery

Here’s a quick comparison of these methods:

Method Optimal Timing What It Detects Main Benefit
Relaxin Blood Test Day 28+ Relaxin hormone Confirms true pregnancy
Ultrasound Day 25-30 Embryos & heartbeats Assesses viability
X-Ray Day 55-60 Fetal skeletons Accurate puppy count

Caring for Your Pregnant Dog

If your vet confirms your dog is pregnant, here’s what you need to know about proper care throughout pregnancy:

Nutrition Through Pregnancy

  • First trimester (Days 1-21): Maintain normal adult diet
  • Second trimester (Days 22-42): Gradually increase food by ~10%
  • Third trimester (Days 43-63): Increase to 30-50% above pre-pregnancy levels

A common mistake many of us make is overfeeding too early or adding calcium supplements. Contrary to popular belief, calcium supplementation during pregnancy is not recommended and can actually increase the risk of eclampsia (milk fever) after whelping.

Exercise Adjustments

  • First trimester: Continue regular activity, but avoid extreme temperatures
  • Second trimester: Maintain regular but moderate exercise, decrease duration
  • Third trimester: Switch to multiple shorter walks, let dog set the pace

Whelping Preparation

Start preparing a whelping area by day 56 with:

  • A quiet location away from household traffic
  • A proper whelping box with “pig rails” to prevent puppy crushing
  • Appropriate bedding that’s washable and non-fraying
  • A heat source for newborn puppies
  • Basic supplies like clean towels, digital scale, and bulb syringe

When to Call the Vet Immediately

Some warning signs during pregnancy that warrant immediate veterinary attention include:

  • Vaginal discharge (especially green, black, or bloody)
  • Severe lethargy or collapse
  • Persistent vomiting or diarrhea
  • Fever (temperature above 102.5°F)
  • During labor: straining without producing a puppy for more than 30-60 minutes
  • More than 2 hours between puppies when you know more are inside

Common Questions About Dog Pregnancy

How long are dogs pregnant?

The average dog gestation period is approximately 63 days (9 weeks) from ovulation, though this can range from 58-68 days from breeding.

Can dogs experience false pregnancy?

Yes! Pseudopregnancy (false pregnancy) is common in dogs and can mimic many pregnancy signs, including behavioral changes, mammary development, and even milk production.

How many puppies can a dog have?

This varies greatly by breed. Small breeds might have 1-4 puppies, while larger breeds can have 8-12 or more. Dogs can potentially have up to 10 litters in their lifetime.

At what age can dogs get pregnant?

Female dogs can become pregnant as early as their first heat cycle, which typically occurs between 6-12 months of age, depending on the breed.

The Bottom Line

While it might be tempting to grab a human pregnancy test from your bathroom cabinet when you suspect your dog is pregnant, the science is clear – it simply won’t work. Dogs and humans have completely different pregnancy hormones, making human tests useless for canine pregnancy detection.

Instead, if you suspect your dog is pregnant, schedule an appointment with your veterinarian for proper testing. The peace of mind and information you’ll gain from professional care is well worth it, and will help ensure both mama dog and potential puppies stay healthy throughout the pregnancy journey.

Have you ever suspected your dog was pregnant? What signs did you notice first? I’d love to hear your experiences in the comments below!


Disclaimer: This article is for informational purposes only and is not a substitute for professional veterinary advice. If you suspect your dog is pregnant, please consult with your veterinarian for proper care and testing.

can you use a pregnancy test on a dog

Bellylabs Pregnancy Test For Dogs

This test is designed to determine pregnancy in bitches and distinguish between pseudopregnancy and actual gestation. It measures the relaxin hormone, a reliable indicator of pregnancy.

The test kit contains all the equipment needed for performing the test at home. No professional knowledge or needles needed.

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